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62 Publications visible to you, out of a total of 62
Multimodal profiling of lung granulomas in macaques reveals cellular correlates of tuberculosis control

Abstract

Not specified

Authors: Hannah P. Gideon, Travis K. Hughes, Constantine N. Tzouanas, Marc H. Wadsworth, Ang Andy Tu, Todd M. Gierahn, Joshua M. Peters, Forrest F. Hopkins, Jun-Rong Wei, Conner Kummerlowe, Nicole L. Grant, Kievershen Nargan, Jia Yao Phuah, H. Jacob Borish, Pauline Maiello, Alexander G. White, Caylin G. Winchell, Sarah K. Nyquist, Sharie Keanne C. Ganchua, Amy Myers, Kush V. Patel, Cassaundra L. Ameel, Catherine T. Cochran, Samira Ibrahim, Jaime A. Tomko, Lonnie James Frye, Jacob M. Rosenberg, Angela Shih, Michael Chao, Edwin Klein, Charles A. Scanga, Jose Ordovas-Montanes, Bonnie Berger, Joshua T. Mattila, Rajhmun Madansein, J. Christopher Love, Philana Ling Lin, Alasdair Leslie, Samuel M. Behar, Bryan Bryson, JoAnne L. Flynn, Sarah M. Fortune, Alex K. Shalek

Date Published: 1st May 2022

Publication Type: Journal

Robust IgM responses following intravenous vaccination with Bacille Calmette–Guérin associate with prevention of Mycobacterium tuberculosis infection in macaques

Abstract

Not specified

Authors: Edward B. Irvine, Anthony O’Neil, Patricia A. Darrah, Sally Shin, Alok Choudhary, Wenjun Li, William Honnen, Smriti Mehra, Deepak Kaushal, Hannah Priyadarshini Gideon, JoAnne L. Flynn, Mario Roederer, Robert A. Seder, Abraham Pinter, Sarah Fortune, Galit Alter

Date Published: 22nd Nov 2021

Publication Type: Journal

CometChip enables parallel analysis of multiple DNA repair activities.

Abstract (Expand)

DNA damage can be cytotoxic and mutagenic, and it is directly linked to aging, cancer, and other diseases. To counteract the deleterious effects of DNA damage, cells have evolved highly conserved DNA … repair pathways. Many commonly used DNA repair assays are relatively low throughput and are limited to analysis of one protein or one pathway. Here, we have explored the capacity of the CometChip platform for parallel analysis of multiple DNA repair activities. Taking advantage of the versatility of the traditional comet assay and leveraging micropatterning techniques, the CometChip platform offers increased throughput and sensitivity compared to the traditional comet assay. By exposing cells to DNA damaging agents that create substrates of Base Excision Repair, Nucleotide Excision Repair, and Non-Homologous End Joining, we show that the CometChip is an effective method for assessing repair deficiencies in all three pathways. With these applications of the CometChip platform, we expand the utility of the comet assay for precise, high-throughput, parallel analysis of multiple DNA repair activities.

Authors: J. Ge, L. P. Ngo, S. Kaushal, I. J. Tay, E. Thadhani, J. E. Kay, P. Mazzucato, D. N. Chow, J. L. Fessler, D. M. Weingeist, R. W. Sobol, L. D. Samson, S. R. Floyd, B. P. Engelward

Date Published: 9th Aug 2021

Publication Type: Journal

Immune cells in bronchoalveolar lavage fluid of Ugandan adults who resist versus those who develop latent Mycobacterium tuberculosis infection

Abstract

Not specified

Authors: Bonnie A. Thiel, William Worodria, Sophie Nalukwago, Mary Nsereko, Ingvar Sanyu, Lalitha Rejani, Josephine Zawedde, David H. Canaday, Catherine M. Stein, Keith A. Chervenak, LaShaunda L. Malone, Ronald Kiyemba, Richard F. Silver, John L. Johnson, Harriet Mayanja-Kizza, W. Henry Boom

Date Published: 1st Apr 2021

Publication Type: Journal

Excision of mutagenic replication-blocking lesions suppresses cancer but promotes cytotoxicity and lethality in nitrosamine-exposed mice.

Abstract (Expand)

N-Nitrosodimethylamine (NDMA) is a DNA-methylating agent that has been discovered to contaminate water, food, and drugs. The alkyladenine DNA glycosylase (AAG) removes methylated bases to initiate the … base excision repair (BER) pathway. To understand how gene-environment interactions impact disease susceptibility, we study Aag-knockout (Aag(-/-)) and Aag-overexpressing mice that harbor increased levels of either replication-blocking lesions (3-methyladenine [3MeA]) or strand breaks (BER intermediates), respectively. Remarkably, the disease outcome switches from cancer to lethality simply by changing AAG levels. To understand the underlying basis for this observation, we integrate a suite of molecular, cellular, and physiological analyses. We find that unrepaired 3MeA is somewhat toxic, but highly mutagenic (promoting cancer), whereas excess strand breaks are poorly mutagenic and highly toxic (suppressing cancer and promoting lethality). We demonstrate that the levels of a single DNA repair protein tip the balance between blocks and breaks and thus dictate the disease consequences of DNA damage.

Authors: J. E. Kay, J. J. Corrigan, A. L. Armijo, I. S. Nazari, I. N. Kohale, D. K. Torous, S. L. Avlasevich, R. G. Croy, D. N. Wadduwage, S. E. Carrasco, S. D. Dertinger, F. M. White, J. M. Essigmann, L. D. Samson, B. P. Engelward

Date Published: 16th Mar 2021

Publication Type: Journal

CD4+ T cells re-wire granuloma cellularity and regulatory networks to promote immunomodulation following Mtb reinfection

Abstract

Not specified

Authors: Joshua D. Bromley, Sharie Keanne C. Ganchua, Sarah K. Nyquist, Pauline Maiello, Michael Chao, H. Jacob Borish, Mark Rodgers, Jaime Tomko, Kara Kracinovsky, Douaa Mugahid, Son Nguyen, Qianchang Dennis Wang, Jacob M. Rosenberg, Edwin C. Klein, Hannah P. Gideon, Roisin Floyd-O’Sullivan, Bonnie Berger, Charles A. Scanga, Philana Ling Lin, Sarah M. Fortune, Alex K. Shalek, JoAnne L. Flynn

Date Published: No date defined

Publication Type: Journal

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