Griffith

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Auto-created investigation: Griffith

SEEK ID: https://nextseek-dev.mit.edu/investigations/2

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Created: 21st Jan 2026 at 15:07

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Studies (2)
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SOPs (9)
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Institutions: BMC-BioMicro Center (MIT)

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Integrating endometrial proteomic and single cell transcriptomic pipelines reveals distinct menstrual cycle and endometriosis-associated molecular profiles

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Lauren Baugh, Brittany A. Goods, Juan S. Gnecco, Yunbeen Bae, Michael Retchin, Constantine N. Tzouanas, Megan Loring, Keith Isaacson, Alex K. Shalek, Douglas Lauffenburger, Linda Griffith

https://www.medrxiv.org/content/10.1101/2022.01.29.22269829v1

Endometriosis is a debilitating gynecological disorder affecting approximately 10% of the female population. Despite its prevalence, robust methods to classify and treat endometriosis remain elusive. Changes ...

Submitter: Demo Demo

Investigation: Griffith

Assays: DNA Extraction - Metadata, Immunohistochemistry - Data Linked, Linear Mixed Model - Data Linked, Mass Spectrometry Proteomics - Data Linked, Mass Spectrometry Proteomics Analysis - Data Linked, Patient Visit - Metadata, Single Cell Expression Matrix Analysis - Data Linked, Single Cell Sequencing - Data Linked, Tissue Collection - Metadata

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Created: 21st Jan 2026 at 15:07

Organoid co-culture model of the cycling human endometrium in a fully-defined synthetic extracellular matrix reveals epithelial-stromal crosstalk

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Juan S. Gnecco, Alexander Brown, Kira Buttrey, Clara Ives, Brittany A. Goods, Lauren Baugh, Victor Hernandez-Gordillo, Megan Loring, Keith Isaacson, Linda G. Griffith

https://doi.org/10.1101/2021.09.30.462577

The human endometrium undergoes recurring cycles of growth, differentiation, and breakdown in response to sex hormones. Dysregulation of epithelial-stromal communication during hormone cycles is linked to myriad gynecological disorders for which treatments ...

Submitter: Demo Demo

Investigation: Griffith

Assays: Cell Culture Imaging - Data Linked, Cell Culture and Organoid Generation - Metadata, DNA Extraction - Metadata, Elisa - Data Linked, Gene Expression Analysis - Data Linked, Immunohistochemistry - Data Linked, Luminex - Data Linked, Mass Spectrometry Proteomics - Data Linked, Patient Visit - Metadata, Short Read Sequencing - Data Linked, Tissue Collection - Metadata

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Created: 21st Jan 2026 at 15:07

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Patient Visit - Metadata

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Investigation: Griffith

Study: Organoid co-culture model of the cycling human ...

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Created: 21st Jan 2026 at 15:08

Tissue Collection - Metadata

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Investigation: Griffith

Study: Organoid co-culture model of the cycling human ...

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Created: 21st Jan 2026 at 15:08

Cell Culture and Organoid Generation - Metadata

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Study: Organoid co-culture model of the cycling human ...

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SOPs: P.GRI-231030-V1_Cell_Culture_and_Organoid_Gener...

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Created: 21st Jan 2026 at 15:08, Last updated: 22nd Jan 2026 at 16:22

DNA Extraction - Metadata

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Study: Organoid co-culture model of the cycling human ...

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SOPs: P.GRI-231101-V1_RNA-isolation-and-bulk-RNA-seq

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Created: 21st Jan 2026 at 15:08, Last updated: 22nd Jan 2026 at 16:46

Immunohistochemistry - Data Linked

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Investigation: Griffith

Study: Organoid co-culture model of the cycling human ...

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Created: 21st Jan 2026 at 15:08

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P.GRI-231101-V1_RNA-isolation-and-bulk-RNA-seq

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Studies: Organoid co-culture model of the cycling human ...

Assays: DNA Extraction - Metadata, Short Read Sequencing - Data Linked

Created: 22nd Jan 2026 at 16:46, Last updated: 22nd Jan 2026 at 16:46

P.GRI-231107-V1_Bulk-RNAseq-analysis-of-in-vitro-co-cultures.docx

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Studies: Organoid co-culture model of the cycling human ...

Assays: Gene Expression Analysis - Data Linked

Created: 22nd Jan 2026 at 16:22, Last updated: 22nd Jan 2026 at 16:22

P.GRI-231030-V1_Cell_Culture_and_Organoid_Generation_Protocol.docx

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Studies: Organoid co-culture model of the cycling human ...

Assays: Cell Culture and Organoid Generation - Metadata

Created: 22nd Jan 2026 at 16:22, Last updated: 22nd Jan 2026 at 16:22

P.GRI-231107-V1_Computational-analyses-of-scRNA-seq-data.docx

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Studies: Integrating endometrial proteomic and single ce...

Assays: Single Cell Expression Matrix Analysis - Data L...

Created: 22nd Jan 2026 at 16:16, Last updated: 22nd Jan 2026 at 16:16

P.GRI-231103-V1_ELISA_Luminex_Protocol

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Studies: Organoid co-culture model of the cycling human ...

Assays: Elisa - Data Linked

Created: 22nd Jan 2026 at 16:10, Last updated: 22nd Jan 2026 at 16:10

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Organoid co-culture model of the human endometrium in a fully synthetic extracellular matrix enables the study of epithelial-stromal crosstalk.

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BACKGROUND: The human endometrium undergoes recurring cycles of growth, differentiation, and breakdown in response to sex hormones. Dysregulation of epithelial-stromal communication during hormone-mediated … signaling may be linked to myriad gynecological disorders for which treatments remain inadequate. Here, we describe a completely defined, synthetic extracellular matrix that enables co-culture of human endometrial epithelial and stromal cells in a manner that captures healthy and disease states across a simulated menstrual cycle. METHODS: We parsed cycle-dependent endometrial integrin expression and matrix composition to define candidate cell-matrix interaction cues for inclusion in a polyethylene glycol (PEG)-based hydrogel crosslinked with matrix metalloproteinase-labile peptides. We semi-empirically screened a parameter space of biophysical and molecular features representative of the endometrium to define compositions suitable for hormone-driven expansion and differentiation of epithelial organoids, stromal cells, and co-cultures of the two cell types. FINDINGS: Each cell type exhibited characteristic morphological and molecular responses to hormone changes when co-encapsulated in hydrogels tuned to a stiffness regime similar to the native tissue and functionalized with a collagen-derived adhesion peptide (GFOGER) and a fibronectin-derived peptide (PHSRN-K-RGD). Analysis of cell-cell crosstalk during interleukin 1B (IL1B)-induced inflammation revealed dysregulation of epithelial proliferation mediated by stromal cells. CONCLUSIONS: Altogether, we demonstrate the development of a fully synthetic matrix to sustain the dynamic changes of the endometrial microenvironment and support its applications to understand menstrual health and endometriotic diseases. FUNDING: This work was supported by The John and Karine Begg Foundation, the Manton Foundation, and NIH U01 (EB029132).

Authors: J. S. Gnecco, A. Brown, K. Buttrey, C. Ives, B. A. Goods, L. Baugh, V. Hernandez-Gordillo, M. Loring, K. B. Isaacson, L. G. Griffith

Date Published: 11th Aug 2023

Publication Type: Journal Article

PubMed ID: 37572651

DOI: 10.1016/j.medj.2023.07.004

Citation: Med. 2023 Aug 11;4(8):554-579.e9. doi: 10.1016/j.medj.2023.07.004.

Created: 25th Aug 2026 at 19:02

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